HPLC stands for High-Performance Liquid Chromatography (formerly referred to as High-Pressure Liquid Chromatography).
It is a chromatographic technique used to separate the components in a mixture, to identify each component, and to quantify each component.
In general, the method involves a liquid sample being passed over a solid adsorbent material packed into a column using a flow of liquid solvent. Each analyte in the sample interacts slightly differently with the adsorbent material, thus retarding the flow of the analytes. If the interaction is weak, the analytes flow off the column in a short amount of time, and if the interaction is strong, then the elution time is long.
If you are applying for a job in this industry then you have to be prepared for the very serious emplyment interview that will take place.
If the interview is conducted for quality control in the pharma or research and development department, then HPLC interview questions and answers are a must. HPLC is an instrument that separates the different components from the solution mixture by using two different phases i.e. stationary phase and mobile phase.
Some basic HPLC interview questions and answers are collected. Here we will understand all those interview questions for HPLC.
Question : What is the Full form of HPLC?
Answer: HPLC stands for High-performance Liquid Chromatography or High-Pressure Liquid Chromatography.
Question : What is the HPLC Principle? or What is the Principle of HPLC Chromatography?
Answer: It is a chromatographic technique used to separate the components from a mixture of a solution by using a solid stationary phase and a liquid mobile phase.
Question : What is the General chapter number of Chromatography?
Answer: USP General chapter Number <621>
Question : How many types of chromatography?
Answer: There are 12 types of chromatography.
Question : What are the names of different types of chromatography?
Answer:
-Gas Chromatography (GC)
-High-Pressure Liquid Chromatography (HPLC)
-Thin Layer Chromatography (TLC)
-Paper Chromatography
-Column Chromatography
-Affinity Chromatography
-Fast Protein Liquid Chromatography
-Supercritical Fluid Chromatography
-Reverse-phase Chromatography
-Countercurrent Chromatography
-Two Dimensional Chromatography
-Pyrolysis Gas Chromatography
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